Evaluation of the Physiological Relationship Between Oxidative Stress and Metabolic Characters: Insights From Hybridization Between Anarhichas minor × A. lupus

C.M. Mavreas1, §, N.R. Le François1, 2, §, H. Tveiten3, P.U. Blier1, *
1 Laboratoire de biologie intégrative, Département de Biologie, Chimie et Géographie, Université du Québec à Rimouski, 300 Allée des Ursulines, Rimouski, QC, Canada G5L 3A1
2 Division des Collections vivantes et de la recherche, Biodôme de Montréal, 4777, Avenue Pierre-De Coubertin, Montréal, QC H1V 1B3
3 Nofima Marine A/S, Muninbakken, Breivika, Tromsø, Norway

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© 2013 Mavreas et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Université du Québec à Rimouski, 300 Allée des Ursulines, Rimouski, QC, Canada G5L 3A1; Tel: 1-418-723-1986 ext. 1852, Fax: 1-418-724-1849 E-mail:
§ Both authors contribute equally


Physiological indicators such as lipid peroxidation and enzymes involved in aerobic/anaerobic energy metabolism were measured in pure and hybrid lines of wolffish juveniles (Anarhichas minor, ♀ A. minor ⨯ ♀ A. lupus). Interspecific hybridization was introduced as a powerful tool to promote augmentation of phenotypic variability and consequently improve our capacity for the detection of clear physiological relationships amongst oxidative stress and metabolic characters. Mitochondrial sequence genes analysis between the Atlantic (A. lupus) and spotted wolffish (A. minor) were later performed to further assess the extent of divergence between A. lupus and A. minor mitochondrial haplotypes. The observed divergences (2.0% and the number of nonsynonymous substitution n=23) do not seem to be sufficient to generate detectable differences in ETS complexes catalytic capacities. Total LDH activity was however higher in hybrids than in the pure strands and tightly correlated to TBARS levels which suggest that LDH actively participates to the regulation of cellular redox status. Our results are discussed in comparison with a similar study on a charr hybrid (Salvelinus alpinus × S. fontinalis).

Keywords: Anarhichas, comparative sequence analysis, energy metabolism, energy metabolism, hybridization, oxidative stress, ROS, spotted wolffish.