RESEARCH ARTICLE


Partial cDNA Sequence Analysis of Myosin Va from Rainbow Trout (Oncorhynchus mykiss) and Its Relationship to Myosin V Isoforms from Other Vertebrates



Kátia Gisele Oliveira Rancura1, Cesar Martins1, *, Michelli Rivero Montaño1, Robson Francisco Carvalho1, Alexandre Azevedo2
1 Departamento de Morfologia, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, São Paulo, Brazil
2 Núcleo de Pesquisas Ecológicas e Sócio Ambientais de Macaé, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Macaé, Rio de Janeiro, Brazil


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© 2008 Rancura et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Cesar Martins, Departamento de Morfologia, Instituto de Biociências, UNESP – Universidade Estadual Paulista, 18618-000, Botucatu, SP, Brazil; Tel/Fax: ++55(14)38116264, E-mail: cmartins@ibb.unesp.br


Abstract

Partial cDNA sequences of myosin V from rainbow trout Oncorhynchus mykiss were analyzed and showed high similarity to MVa from other vertebrates. Phylogenetic analysis has shown that events resulting in the formation of paralogous copies of myosin Va, Vb, and Vc occurred before the divergence of vertebrates into different classes. Expression analysis of myosin Va, Vb, and Vc in different O. mykiss tissues revealed MVa exclusively expressed in hypophysis and brain whereas Vb and Vc were expressed in practically all tissues analyzed. The nucleotide sequence for myosin V was explored in a fish species for the first time and these results represent an important start in understanding the organization, evolution, and expression of myosins in early vertebrates. The data presented here represent contributions to the knowledge of rainbow trout genome. A better understanding of this economically important species could assist in development of improved strains of this fish for aquaculture.

Keywords: Nucleotide sequence, Amino acid sequence, Evolution, Tissue expression, Fish.