Partial cDNA Sequence Analysis of Myosin Va from Rainbow Trout (Oncorhynchus mykiss) and Its Relationship to Myosin V Isoforms from Other Vertebrates
Kátia Gisele Oliveira Rancura1, Cesar Martins1, *, Michelli Rivero Montaño1, Robson Francisco Carvalho1, Alexandre Azevedo2
Identifiers and Pagination:Year: 2008
First Page: 1
Last Page: 7
Publisher Id: TOFISHSJ-1-1
Article History:Received Date: 23/01/2008
Revision Received Date: 01/02/2008
Acceptance Date: 07/02/2008
Electronic publication date: 10/3/2008
Collection year: 2008
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Partial cDNA sequences of myosin V from rainbow trout Oncorhynchus mykiss were analyzed and showed high similarity to MVa from other vertebrates. Phylogenetic analysis has shown that events resulting in the formation of paralogous copies of myosin Va, Vb, and Vc occurred before the divergence of vertebrates into different classes. Expression analysis of myosin Va, Vb, and Vc in different O. mykiss tissues revealed MVa exclusively expressed in hypophysis and brain whereas Vb and Vc were expressed in practically all tissues analyzed. The nucleotide sequence for myosin V was explored in a fish species for the first time and these results represent an important start in understanding the organization, evolution, and expression of myosins in early vertebrates. The data presented here represent contributions to the knowledge of rainbow trout genome. A better understanding of this economically important species could assist in development of improved strains of this fish for aquaculture.